Fig. 6

daf-16 is essential for freeze–thaw stress survival. daf-16 RNAi and daf-16-defective mutant loss enhanced survival of insulin signaling-defective mutants, including daf-2(e1370), age-1(hx546), and pdk-1(sa709), and wild-type (N2). daf-18-defective mutants also had decreased stress survival after freeze–thaw treatment, similar to daf-16 RNAi mutants. Every assay was repeated at least three times (n > 80–227) in the same conditions. Statistical significance was assessed by the Mann–Whitney test. **p < 0.01, n.s. - not significant