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Table 3 Least square estimated mean values for tested traits by diet and sex and their interaction. Dietary challenge from age 8–20 weeks. C, Chow diet (18 % fat); H, High fat diet (42 % fat); M, male; F, female; CM, males on chow diet; CF, females on Chow diet; HM, males on high-fat diet; HF, females on high-fat diet. Above, estimated mean; in parentheses below, standard error (SE). Values in the same row that share the same superscript letter do not differ significantly at P < 0.05. GxD, significance of Sex x Diet interaction. RIF, increase on HFD relative to Chow, females; RIM, increase on HFD relative to Chow, males. NS, not significant. ND, not done, as data are prior to HFD treatment e. BW, Body Weight; BMI, Body Mass Index; WC, Waist circumference; Total AUC, total area under curve of the intraperitoneal glucose tolerance test; Initial, at experiment start-point age of 8 weeks; End, end time-point of the experiment after 12 weeks high-fat dietary challenge; Gain, difference between end and initial time-point values

From: High-fat-diet induced development of increased fasting glucose levels and impaired response to intraperitoneal glucose challenge in the collaborative cross mouse genetic reference population

Trait

CF

HF

CM

HM

SXD

RIF

RIM

RIM/RIF

BW Initial

18.87a

18.92a

22.99b

22.92b

    

(0.31)

(0.27)

(0.24)

(0.24)

NS

ND

ND

ND

BMI Initial

0.218a

0.216a

0.244b

0.243b

NS

ND

ND

ND

(0.005)

(0.004)

(0.004)

(0.003)

    

WC Initial

5.90a

5.86a

6.69b

6.64b

 

ND

ND

ND

(0.11)

(0.1)

(0.09)

(0.09)

NS

BW End

23.44a

25.52b

28.70c

34.35d

    

(0.65)

(0.55)

(0.5)

(0.49)

***

1.089

1.197

1.099

BMI End

0.230a

0.230a

0.260b

0.310c

    

(0.006)

(0.005)

(0.005)

(0.004)

***

1

1.192

1.192

WC End

6.77a

6.58a

7.92b

8.74c

    

(0.17)

(0.15)

(0.13)

(0.13)

***

0.927

1.103

1.19

BW Gain

4.27a

6.53c

5.70bc

11.42d

    

(0.56)

(0.48)

(0.43)

(0.42)

***

1.529

2.004

1.31

BMI Gain

0.011a

0.014a

0.020a

0.063b

    

(0.007)

(0.006)

(0.005)

(0.005)

***

1.273

3.15

2.474

WC Gain

0.87ab

0.72a

1.23b

2.09c

    

(0.18)

(0.15)

(0.14)

(0.14)

***

0.827

1.699

2.055

Fasting Glucose

146.15a

176.26c

163.17bc

206.58d

    

(3.86)

(6.65)

(3.35)

(5.62)

NS

1.206

1.266

1.05

Total AUC

25701.2a

37497c

32923.1b

53004.9d

    

(1126.7)

(1224.4)

(1069.7)

(1080.4)

***

1.459

1.61

1.103

  1. e No, of animals: for BW, BMI, WC: Chow Diet; females 16 lines, 85 animals; males, 20 lines 141 animals; HFD, females 21 lines 116 animals, males 21 lines 149 animals. For Fasting Glucose and AUC: Chow diet, 17 lines 137 females, 146 males; HFD 21 lines, 116 females, 149 males
  2. ***, P < 0.001