Table 1 Primer pairs used to amplify the ε-LCY and β-LCY genes. The final two columns give the size of amplicons and the used high-throughput technology (CelI/agarose gel or DHPLC method). The partial denaturation DHPLC temperatures are reported in brackets

β-LCY-6A

A

1

F4f

AGCCCTACAACCCGGGA

861

CelI/agarose gel

PC63r

CCCATGAAGATCTTGAGA

F4f

AGCCCTACAACCCGGGA

464

DHPLC (65.8 °C)

OP6r

GTGCGCGCCACCATGTACC

β-LCY-6B

B

1

PC71f

ATCCCGGCCACCGTCGTCCTGGA

990

CelI/agarose gel

PC62r

CCATGAAGATCTTGAGATGC

PC68f

GTCTTCATCGACGACCACA

761

DHPLC (65.1 °C)

OP6

GTGCGCGCCACCATGTACC

ε-Lcy-3A

A

4–9

PC35f

TGCTGAGAAGGTAGACATTCTATTG

1,193

CelI/agarose gel

PC40r

CAAGCATTGATGGACTGGAC

4–5

PC35f^a

TGCTGAGAAGGTAGACATTCTATTG

650

DHPLC (57.8 °C)

PC130r^a

CATTGCAGAAGCACACTGC

6–7–8

PC42f^a

GGTTGATGTCTCGGTTGGAT

443

DHPLC (57.9 °C)

PC40r^a

CAAGCATTGATGGACTGGAC

8–9

PC43f

TGGACAATATTTGCCTGGAA

385

DHPLC (57.5 °C)

PC37r

CTTGCGTACTCGCGAAAAA

PC35f

TGCTGAGAAGGTAGACATTCTATTG

1,527

Used for nested PCR

PC37r

CTTGCGTACTCGCGAAAAA

ε-Lcy-3B

B

4–9

PC42f

GGTTGATGTCTCGGTTGGAT

1,530

CelI/agarose gel

PC46r

GCATCCTTGCGTATTGTATTCTT

4

PC44f^a

TTGCTGAGAAGGTACATTCGAT

336

DHPLC (58.4 °C)

PC140r^a

GGCACTTTGTGCAGGGTTGG

5–6–7

PC41f

GAGGACCACGTGTTTGTGTG

584

DHPLC (58.1 °C)

PC143r

ACACCTGTGCAAGATAAACC

7–8–9

PC147f

TCCTTACCTAACACAGACCAGA

636

DHPLC (58.2 °C)

PC48r

AAAGATACGCATCCTTGCGTATT