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Table 5 Mutation frequencies obtained through DHPLC analysis on 1,140 screened plants of the mutagenized durum wheat cv. Marco Aurelio

From: DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population

Gene

Primer

 

Exon region

Amplicon size (bp)

Mutation n°

Mutation frequency (1/kb)

β-LCY-6A

F4f-OP6r

 

1

464

17

 

1/31

β-LCY-6B

PC68f-OP6r

 

1

761

8

 

1/108

ε-LCY-3A

PC35f-PC130r

 

4–5

650

12

1/62

 
 

PC42f-PC40r

 

6–7–8

443

2

1/252

 
 

PC43f-PC37r

 

8–9

384

3

1/146

 
  

mean

    

1/102

ε-LCY-3B

PC44f-PC140r

 

4

336

4

1/95

 
 

PC41f-PC143r

 

5–6–7

584

7

1/95

 
 

PC147f-PC48r

 

7–8–9

636

10

1/72

 
  

mean

    

1/84

Total/mean

   

4,258

46

 

1/77