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Table 2 Protein differentially expressed for Shaziling pig breeds and Yorkshire were identified by 2-DE and MALDI-TOF-MS/MS

From: Integrative analysis of transcriptomics and proteomics of skeletal muscles of the Chinese indigenous Shaziling pig compared with the Yorkshire breed

Sport no NCBI accession number Protein name Mr, kDa theor pI theor No. of peptides identified Mascot score Sequence coverage Expecte
Up-regulated in Yorkshire pigs
 1375 gi|89574051 mitochondrial ATP synthase, H+ transporting F1 complex beta subunit, partial, ATP5B 47059.6 4.99 16 1180 46 % 5.8e-11
 1929 gi|283993079 L-gulonate3-dehydrogenase 35433.2 5.79 14 417 51 % 1.2e-03
 2268 gi|545835136 PREDICTED: NADH dehydrogenase ubiquinone flavoprotein 2 isoform X1 25812.2 6.96 14 435 60 % 1.8e-039
 2247 gi|55926209 heat shock protein beta-1 22984.7 6.23 9 423 41 % 2.9e-03
 2393 gi|809283 Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution 16082.4 6.76 12 511 86 % 4.6e-04
 2377 gi|545848507 PREDICTED: alpha-crystallin B chain isoform X2 20116.4 6.76 9 212 51 % 3.7e-01
 2497 gi|494389 Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin 16901.8 6.5 9 529 59 % 7.3e-04
 2500 gi|494389 Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin 16901.8 6.5 12 596 82 % 1.5e-05
 2575 gi|55926217 cytochrome c oxidase subunit 5B, mitochondrial precursor 14002 8.8 8 334 46 % 2.3e-02
 2594 gi|809283 Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution 16082.4 6.76 9 400 67 % 5.8e-03
Up-regulated in Shaziling pigs
 2480 gi|117660856 MYLPF 19.0 4.8 6 110 36 % 5.8e-00
 2395 gi|117660874 MLC1f 21018.6 4.9 11 444 60 % 2.3e-40
 2654 gi|117660856 MYLPF 19066.3 4.8 9 347 49 % 1.2e-03
 2422 gi|117660890 MLC3f 16761.2 4.6 4 250 29 % 2.3e-02
 2659 gi|117660856 MYLPF 19066.3 4.8 6 144 27 % 2.3e-01
 2400 gi|545858131 keratin, type I cytoskeletal 10 isoform X2 58318.6 4.9 16 190 21 % 5.8e-01
 2482 gi|117660856 MYLPF 19066.3 4.8 16 600 86 % 5.8e-05
 2522 gi|117660874 MLC1f 21018.6 4.9 8 190 40 % 5.8e-01
 1154 gi|545845559 PREDICTED: fibrinogen beta chain isoform X2 50399.8 7.94 18 gi|545845559 43 % 4.6e-03
 2225 gi|262263205 triosephosphate isomerase 1 26878.9 6.54 14 463 74 % 2.9e-04
 2203 gi|262263205 Triosephosphate somerase 1 26878.9 6.54 17 774 77 % 2.3e-07
 1326 gi|311247991 PREDICTED: pyruvate dehydrogenase protein Xcomponent-like isoform 54036.4 8.3 12 317 28 % 1.2e-02
 1416 gi|545801458 PREDICTED: actin, alpha cardiac muscle 1 isoform X1 42334 5.2 10 313 31 % 2.9e-02
 2250 gi|262263205 Triosephosphate isomerase 1,TPI1 26878.9 6.5 17 795 82 % 1.8e-07
 2584 gi|314907119 A-FABP adipocyte fatty acid-binding protein 14780.6 6.2 8 371 56 % 4.6e-03
 1433 gi|545833443 PREDICTED: alpha-enolase isoform X1 38172.7 8.93 12 377 41 % 1.2e-03
 2221 gi|55926209 HSPB1 heat shock protein beta-1 22984.7 6.23 8 236 31 % 1.5e-01
 1805 gi|545859898 PREDICTED: beta-enolase isoform X1 48154.9 8.52 17 414 40 % 2.3e-03
 2550 gi|545805333 PREDICTED: 14 kDa phosphohistidine phosphatase isoform X2 14036.8 5.91 14 454 74 % 2.3e-04
 2217 gi|262263205 triosephosphate isomerase 1 26878.9 6.54 16 701 82 % 4.6e-06
 1711 gi|46389777 troponin T fast skeletal muscle type 30720.1 8.68 14 423 41 % 2.9e-03
 1137 gi|545845559 PREDICTED: fibrinogen beta chain isoform X2 50399.8 7.94 15 289 34 % 7.3e-02
 2215 gi|262263205 triosephosphate isomerase 1 26878.9 6.54 18 684 76 % 2.3e-06
 2223 gi|55926209 HSPB1 heat shock protein beta-1 22984.7 6.23 10 371 42 % 4.6e-03
 1833 gi|545832797 PREDICTED: troponin T, slow skeletal muscle isoformX1 32449.6 5.54 9 418 32 % 9.2e-03
 1374 gi|268607671 actin, alpha skeletal muscle 42366 5.23 11 348 35 % 9.2e-03
  1. eNumber of times we would expect to obtain an equal or higher score by chance