Fig. 2From: Multiple regulatory variants located in cell type-specific enhancers within the PKP2 locus form major risk and protective haplotypes for canine atopic dermatitis in German shepherd dogsRegulatory potential and allelic differences of candidate regions. Luciferase reporter assays for each of the eight evaluated SNPs (SNP 27:18,861,228, SNP 27:19,086,778, SNP 27:19,093,355, SNP 27:19,096,199, SNP 27:19,112,169, SNP 27:19,114,170, SNP 27:19,135,677, and SNP 27:19,140,837) were performed in four different cell lines: a. MDCK, b. HaCaT, c. Jurkat, and d. K562. The cloned fragments showed differential activity in different cell lines, indicating the presence of cell type-specific enhancers. The fragment with the SNP 27:19,086,778 contained the most potent enhancer showing the highest activity in HaCaT cells (8 vs. 11-fold induction) with significant allelic difference (p = 0.003). All except SNP 27:19,114,170 showed allelic differences in at least one cell lineBack to article page