Skip to main content

Table 1 Primers for qualitative PCR markers

From: Development of chromosomal markers based on next-generation sequencing: the B chromosome of the cichlid fish Astatotilapia latifasciata as a model

Primer ID

Primer sequence

49B1-F

5′ GAGCTTCACACTTGCAGAGGTAAGTCATTTTTGCAGAGAC 3′

49B2-F

5′ GCTTCACACTTGCAGAGGTAAGTCATTTTT 3′

49C + −F

5′ GTTTACAGTCTGATGATGGGACATCATGCTCTGC 3′

49-R

5′ TGTCCAGAGTATAATCGCAGCCTTTGCGGT 3′

80B-F

5′ GAGGCATTACATCGGTCTTTCCATCA 3′

80C + −F

5′ GGTGAGCAGCAGGATTTTGAATTGAATGCG 3′

80-R

5′ CCTGATTGAGTGCTTCTCACAC 3′

182B1-F

5′ GGGTGTGTTTGGTTGTGGTTTGACAAGGAGTG 3′

182B2-F

5′ GGAGTGAATTGTGATGGT 3′

182B3-F

5′ GGTTTGACAAGGAGTGAATTGTGATGGTTAGATC 3′

182B4-F

5′ GAGTGAATTGTGATGGTTAGATCACTAGGTAT 3′

182C + −F

5′ AGAATGGTCCAAGGAAGG 3′

182-R

5′ CCATCAGAACCAGCATTAA 3′

207B-F

5′ GAGACACTTCTTGGAGAAAATGAAATGCCCAC 3′

207C + −F

5′ ACCAGGCCAGGAGACGACTGAAGAACT 3′

207-R

5′ GACCTGCAGAAATGTGAACATGGTTGCAGTTTACAA 3′

323B-F

5′ GGGGGTGTTTTGCTTTTGGTTTTCCTACATTAGTTA 3′

323C + −F

5′ GTATAAGCCATCTCTGTCATCTAAGGTACA 3′

323-R

5′ GACACAGTACAGCTGACACAGACGAAGCAACAG 3′

764B-F

5′ CCTGAGATGGTCCGATTGGGCTGGTAA 3′

764C + −F

5′ GGTGAAGCATCAAAGAGCTCTCTGAGTCT 3′

764-R

5′ GGAGACAAGGAGATGCGTGTTGGTGAAGTCCTAA 3′

1100B-F

5′ GGGTGTGTGGAGATGTACATCAGCACACATGTT 3′

1100C + −F

5′ CACTGAGACGGCATTGGCATGAGAAA 3′

1100-R

5′ AGCATGGTGGCAGAGGTCTTTA 3′

1987B-F

5′ CCCTCCTGTTATTCATTCCCTA 3′

1987C + −F

5′ TACTTTGCTGTGTGTTTTGCCTGTC 3′

1987-R

5′ AAGTGTGGCTGTGTGCAGGCAGGAAT 3′

2519B-F

5′ GCAGGATTCAGGAGTGAAGCATCTGTGTGA 3′

2519C + −F

5′ CACTAAACTGCAGACATCAGGCTG 3′

2519-R

5′ CATTGTTCTGCTGCAGTCAATGGAC 3′

  1. The numbers 49, 80, 182, 207, 323, 1100 and 1987 are references to the contigs obtained with 454 sequencing data
  2. The letters B and C indicate the specificity of the primer as a B-specific primer or positive control (B or C+), respectively, followed by the reverse/forward annotation (F or R)