Table 3 qPCR primer set designed over six high-coverage genomic regions
Primer ID | Primer sequence |
|---|---|
Scaffold_3-F | 5′ GCCACCATGTTCAGATTATTGGAGAGTA 3′ |
Scaffold_3-R | 5′ AATGCCTGACTTATCCATGCCAGGTG 3′ |
Scaffold_13-F | 5′ CGTTTTGTACGTCTGCTGGA 3′ |
Scaffold_13-R | 5′ ACCGGTACCTGTGGTCTAGT 3′ |
Scaffold_19-F | 5′ TGGAGCATGAGTCGAAAAGCA 3′ |
Scaffold_19-R | 5′ TCGCAGAACAGTGTGAACCA 3′ |
Scaffold_26-F | 5′ AGACGGGTCGGGATCTTACA 3′ |
Scaffold_26-R | 5′ TGTTTGAGCATCCCCCAGAC 3′ |
Scaffold_31-F | 5′ CCAAGGCTCAGGAAATAGGGG 3′ |
Scaffold_31-R | 5′ ACCACTGCTTCTCAAAGAGGG 3′ |
Scaffold_324-F | 5′ CAGGTCCCTCTGCGTAACTG 3′ |
Scaffold_324-R | 5′ GACGCCCCAGTCATCATTCA 3′ |