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Fig. 3 | BMC Genetics

Fig. 3

From: Growth conditions that increase or decrease lifespan in Saccharomyces cerevisiae lead to corresponding decreases or increases in rates of interstitial deletions and non-reciprocal translocations

Fig. 3

No correlation between recombination, retrotransposition, or CAN1 mutations and lifespan-altering treatments in strain SCE218. a Assay for recombination between two truncated alleles of the LEU2 gene with overlapping sequences that removes intervening plasmid sequences and restores a wild type LEU2 allele, conferring a Leu+ prototroph phenotype. b Assay for retromobility of a chromosomal Ty1his3AI element. Expression of Ty1his3AI allows splicing of an artificial intron present in the opposite transcriptional orientation of the HIS3 gene, reverse transcription, and insertion of a Ty1HIS3 cDNA into the genome, conferring a His+ prototroph phenotype. (c) Rate values for the indicated forms of genomic instability obtained from fluctuation tests for cells grown for three days in control YPD broth at 20 °C or using the indicated conditions that shortened (bold) or lengthened (gray) lifespan in the SCE218 strain. Mean and standard deviation for four control trials and two trials in each alternative condition are shown. Asterisks indicate level of significance: * = p < 0.05, ** = p < 0.01

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