Fig. 4

CAN1 mutation rates in strain JC5516 are strongly correlated with lifespan-altering treatments. a Illustration of the relative positions of URA3, HIS3, and CAN1 genes inserted onto the distal portion of the right arm of chromosome VIII in the JC5516 strain. b CAN1 mutation rates after growth for three days in control YPD broth at 20 °C or in the indicated growth conditions that were found to shorten lifespan (bold) or lengthen lifespan (gray) of strain JC5516. c URA3 mutation rates after growth for three days in control YPD broth at 20 °C or the same conditions as for panel b. d and e CAN1 d or URA3 e mutation rates after four days of growth in the same conditions as panels a and b with chronic exposure to 200 μM (0.0022 %) MMS. Mean and standard deviations for six or seven trials in YPD and three to five trials in alternative media conditions (b and c) or three to four trials with MMS treatment (d and e) are shown. Note the change in y-axis scales between panels b and d and panels c and e. Asterisks indicate level of significance: * = p < 0.05, ** = p < 0.01