Fig. 6

Loss of CAN1 in JC5516 frequently results from non-reciprocal translocations or interstitial deletions. a Drawings of relative positions and orientations of selected gene, pseudogene, and telomeric sequences (TEL) flanking the site of insertion of CAN1 and HIS3 on the right arm of chromosome VIII (blue), as well as selected features for relevant regions from the right and left arms of chromosome I (orange). Dotted vertical lines with percentages indicate sequence identity between regions of chromosomes VIII and I. Boxed numbers and small arrows indicate the relative positions of representative primers used to test for chromosome VIII-I translocations. Representative images of PCR products from the parent strain (P) and independent can1his3 derivatives visualized by staining agarose gels with ethidium bromide (signal inverted) are shown for the two different pairs of primers. The translocation product is the upper product in the left image, and the lower product was generated with a control primer pair to the HSP104 gene at an unrelated chromosome region. Numbers to the left of the images indicate the positions of molecular mass markers in kbp. b Drawings of the most common non-allelic recombination events identified and their frequency in the total set of events. Gray boxes at the junctions indicate that the precise transitions between FLO5 sequences or sequences between FLO5 and the site of HIS3 on chromosome VIII and chromosome I sequences varied amongst the independent samples. The blue arrow with the gray box for the third class of events indicates a fusion between FLO5 and the YHR213W pseudogene at various sites within each sequence. Sequence features are from the S. cerevisiae reference genome [66]