Table 1 Cotton phytochrome and HY5-specific CAPS and dCAPS markers
# | CAPS markers | Primer sequences (5’–3’) | PCR products (bp) | Restriction enzyme | Restriction products (bp) | |
|---|---|---|---|---|---|---|
G. hirsutum (‘TM-1’) | G. barbadense (‘Pima 3-79’) | |||||
1. | PHYA1_CAPS | F-5’TGCAAAGCAGGAACTTGGCA R-5’CATCCATTTGATAGTCCTTCCAC 3’ | 122 | Mbo I Dpn II | 51/71 | 122 |
2. | PHYB_dCAPS | F-5’CAACCTCAAAATCTGATGAAGTAAAC3’ R-5’CTATCAAAACTCAGAACTGCTAAAGC3’ | 149 | Alu I | 149 | 125/149 |
3. | GhHY5-2_dCAPS | F-5’AACTATATCTGGGAATTACCGATT3’ R-5’GTTTCGCAACAACCTCTTTTCA3’ | 97 | Hinf I | 27/70 | 97 |