Fig. 5

Expression level of putative effector encoding genes from Trichoderma in confrontation with Rhizoctonia solani AG2. a – d, expression level of the selected genes tvlysm1, tvsep3, tvhydii1 and tvmp1 from T. virens. e–g, expression level of the selected genes talysm1, tacfem1 and epl2 from T. atroviride. BC, before contact between both fungi; C, initial contact of the fungi; OG, growth of Trichoderma over R. solani AG2. 2^(−ΔΔCt) values represent the change in the expression level of each gene compared to the control condition (fungus growing alone), which has a designated value of 1 (dotted line). Data were analyzed with a two-way ANOVA and a Bonferroni post hoc test; * p < 0.05; ** p < 0.01; *** p < 0.001. Values represent means of three replicates, bars indicate standard deviation