Fig. 3From: Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stagesReal-time quantitative PCR validation of identified miRNAs using Solexa sequencing technology. The relative quantification of expression was calculated using the 2-∆∆Ct method after the threshold cycle (Ct) and was normalized with the Ct of 18S rRNA. *represents P < 0.05Back to article page