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Fig. 4 | BMC Genetics

Fig. 4

From: Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.)

Fig. 4

pBEGFP-StSnRK2 protein targeted to nucleus and cytoplasm in onion epidermal cells. The pEBGFP and pEBGFP-StSnRK2s plasmids were introduced into Agrobacterium tumefaciens strain EHA105 and were used for transformation into onion epidermal cells. Onion epidermal tissues were subsequently incubated on MS solid nutrient medium in the dark at 26 °C for 48 h. Localization of fluorescent proteins in onion epidermal cells were observed by a Zeiss LSM 710/ConfoCor2 laser-scanning imaging system (CarlZeiss, Jena, Germany). Fluorescence was detected between 505 and 550 nm with excitation at 488 nm. GFP fluorescence and light field vision were recorded in separate channels and then merged into an overlay image. Bar = 50 μm

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