Fig. 3

Knockdown of DmManf increases the number of mitotic cells in the wing disc. a Number of phosphorylated Histone-3 (pHis3) positive cells was significantly increased in GFP-expressing area of wing discs of third instar male larvae when DmManf was knocked down with MS1096-GAL4 in heterozygous DmManf ^Δ96 mutant background. UAS-GFP.nls was used to detect GAL4 expressing area. n = 30. *, P < 0.05, Student’s t-test. b Three representative images of dorsal wing disc area showing pHis3 positive cells (magenta) and MS1096-GAL4 expression with UAS-GFP.nls (green). Scale bar 10 μm. c-d Proportion of S/G2/M positive cells (green) was increased in MS1096-GAL4 expressing wing discs of third instar male larvae in DmManf knockdown in comparison to wild type. act-His2Av-RFP (magenta) was used to mark all nuclei. Representative images of UAS-S/G2/M-Green driven with MS1096-GAL4 in wild type and together with UAS-DmManf-RNAi are shown (d). Boxed magnifications in upper right corners show the 37.9 μm × 37.9 μm area of dorsal wing disc compartment (dashed rectangles in red) used for analysis. Scale bar 10 μm. n = 11 for wt; n = 7 for DmManf-RNAi. **, P < 0.01, Student’s t-test