Fig. 7

The Drosophila homologue of Ku70, Irbp, is a genetic interacting partner of DmManf. a Heterozygous DmManf ^Δ96 mutant background affected phenotype of Irbp knockdown with wing driver MS1096-GAL4 in adult male flies (a3-a4). Scale bar 1 mm. b-c The wing discs of Irbp knockdown with MS1096-GAL4 showed significantly decreased width (red dashed line) in comparison to control genotype (MS1096-GAL4; UAS-mCD8-GFP). n = 8 (control), n = 5 (Irbp-RNAi in wild type background), n = 7 (Irbp-RNAi in heterozygous DmManf ^Δ96 mutant background). **, P < 0.01, ***, P < 0.001, Student’s t-test. n.s., not significant. Scale bar 50 μm. d-e Knockdown embryos of DmManf with 69B-GAL4 showed decreased DmManf and Irbp mRNA levels (d) and Irbp mRNA expression was decreased in 3rd instar larvae of Irbp knockdown with 69B-GAL4 analysed by qPCR (e). Overexpression of DmManf did not affect Irbp mRNA expression. DmManf mRNA expression was not altered in Irbp knockdown with 69B-GAL4. Overexpression of DmManf with 69B-GAL4 increased DmManf mRNA level (relative mRNA expression >30; not shown). *, P < 0.05, **, P < 0.01, ***, P < 0.001, Student’s t-test