Table 3 Associations of 5 SNPs of CDKN1A gene with milk production traits in Chinese Holstein cattle during two lactations (LSM ± SE)

c.271C > T

1

CC(582)

10,249 ± 62.43^a

339.12 ± 2.79

3.32 ± 0.026^ab

302.37 ± 2.03

2.95 ± 0.008

CT(412)

10,212 ± 65.4^Aa

341.67 ± 2.89

3.36 ± 0.027^a

301.44 ± 2.11

2.96 ± 0.009

TT(76)

10,478 ± 99.65^Bb

340.05 ± 4.18

3.27 ± 0.04^b

308.04 ± 3.05

2.95 ± 0.014

P

0.0162*

0.414

0.0377*

0.0522

0.8062

2

CC(394)

10,564 ± 64.48^Aa

378.99 ± 2.86^Aa

3.6 ± 0.027

313.03 ± 2.08^A

2.97 ± 0.009^Aa

CT(302)

10,772 ± 67.72^Bb

387.31 ± 2.98^Bb

3.6 ± 0.028

319.25 ± 2.17^Ba

2.97 ± 0.009^Aa

TT(54)

10,819 ± 119.09^b

388.01 ± 4.94^ab

3.61 ± 0.048

327.12 ± 3.6^Bb

3.03 ± 0.017^B

P

0.0012**

0.0018**

0.9967

<.0001**

0.0012**

c.*9C > G

1

CC(593)

10,229 ± 61.78^a

338.35 ± 2.76

3.33 ± 0.026

301.99 ± 2.01

2.96 ± 0.008

CG(415)

10,173 ± 64.52^Aa

339.45 ± 2.85

3.35 ± 0.027

300.37 ± 2.08

2.96 ± 0.009

GG(76)

10,428 ± 99.99^Bb

338.21 ± 4.19

3.28 ± 0.04

306.56 ± 3.06

2.95 ± 0.014

P

0.0193*

0.8322

0.0956

0.0613

0.8896

2

CC(401)

10,603 ± 64.7^A

380.64 ± 2.87^Aa

3.6 ± 0.027

314.09 ± 2.09^A

2.97 ± 0.009^Aa

CG(305)

10,811 ± 67.06^Ba

389.97 ± 2.96^Bb

3.61 ± 0.028

320.55 ± 2.15^B

2.97 ± 0.009^Aa

GG(54)

10,922 ± 117.94^Ba

391.5 ± 4.89^b

3.59 ± 0.048

329.78 ± 3.56^C

3.02 ± 0.017^B

P

0.0004**

0.0003**

0.874

<.0001**

0.0055**

c.*654C > T

1

CC(497)

10,276 ± 62.96

337.95 ± 2.8

3.3 ± 0.026^Aa

303.16 ± 2.04

2.95 ± 0.008

CT(479)

10,213 ± 63.44

340.69 ± 2.82

3.35 ± 0.026^Bb

301.57 ± 2.05

2.96 ± 0.008

TT(107)

10,298 ± 90.26

338.05 ± 3.83

3.29 ± 0.037^ab

303.44 ± 2.79

2.95 ± 0.013

P

0.3235

0.3355

0.0144*

0.4693

0.7739

2

CC(350)

10,862 ± 65.32

388.92 ± 2.89

3.58 ± 0.027

323.75 ± 2.11^Aa

2.98 ± 0.009

CT(330)

10,755 ± 67.61

389.17 ± 2.98

3.61 ± 0.028

319.07 ± 2.17^Bb

2.96 ± 0.009

TT(79)

10,756 ± 102.96

384.73 ± 4.33

3.58 ± 0.042

316.84 ± 3.16^b

2.95 ± 0.014

P

0.1847

0.529

0.5

0.0092**

0.0922

g.10569766 T > C

1

CC(79)

10,394 ± 98.78

334.84 ± 4.14

3.26 ± 0.04

304.06 ± 3.02

2.94 ± 0.014

CT(427)

10,192 ± 65.04

336.77 ± 2.88

3.32 ± 0.027

299.51 ± 2.09

2.95 ± 0.009

TT(564)

10,191 ± 62.48

335.13 ± 2.79

3.31 ± 0.026

299.53 ± 2.03

2.95 ± 0.008

P

0.0672

0.6615

0.1899

0.2059

0.878

2

CC(60)

10,803 ± 113.4^a

386.17 ± 4.72^ab

3.59 ± 0.046

326.9 ± 3.44^Aa

3.03 ± 0.016^A

CT(309)

10,790 ± 67.29^Aa

388.76 ± 2.96^Aa

3.61 ± 0.028

320.1 ± 2.16^Ab

2.97 ± 0.009^Ba

TT(380)

10,560 ± 65.37^Bb

379.99 ± 2.9^Bb

3.61 ± 0.027

313.48 ± 2.11^B

2.98 ± 0.009^Ba

P

0.0004**

0.0015**

0.8386

<.0001**

0.0009**

g.10569779 T > C

1

CC(559)

10,286 ± 61.82

339.28 ± 2.76

3.32 ± 0.026

303.96 ± 2.01

2.96 ± 0.008

CT(434)

10,251 ± 65.2

339.93 ± 2.89

3.33 ± 0.027

302.21 ± 2.1

2.95 ± 0.009

TT(87)

10,353 ± 95.78

337.22 ± 4.03

3.28 ± 0.039

305.05 ± 2.94

2.96 ± 0.013

P

0.4605

0.7357

0.4159

0.3306

0.7423

2

CC(374)

10,598 ± 64.94^Aa

382.05 ± 2.88^Aa

3.62 ± 0.027

314.19 ± 2.09^A

2.97 ± 0.009^Aa

CT(316)

10,799 ± 66.94^Bb

388.48 ± 2.95^Bb

3.61 ± 0.028

319.81 ± 2.15^Ba

2.97 ± 0.009^Aa

TT(65)

10,726 ± 108.83^ab

381.24 ± 4.54^ab

3.57 ± 0.044

323.07 ± 3.31^Ba

3.02 ± 0.015^B

P

0.0041**

0.0197*

0.6029

0.001**

0.0035**