Table 3 Primers used to determine the relative expression of the PDE3A, PDGFRB and MARCH1 genes as well as GAPDH
Gene name | Primer sequence (5′–3′) | Fragment size (bp) | Annealing temperature (°C) |
|---|---|---|---|
PDE3A | F: TCCCCAGGGAACAGCTCAT | 141 | 60 |
R: CTGCCAGGAGGTCAGTGATG | |||
PDGFRB | F: AGGCATCAGCAGCAAGGATAC | 106 | 60 |
R: CTGTGGTCCCAGCAGAAACA | |||
MARCH1 | F: GCGTGGTCTGGTCCTTGTAT | 84 | 60 |
R: GCCATTCGAGGACACCGTTA | |||
GAPDH | F: AATGGAAAGGCCATCACCATC | 136 | 60 |
R: GTGGTTCACGCCCATCACA |