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Table 6 Haplotype-based association analyses with semen production traits in Chinese Holstein bulls (LSM ± SE)

From: Genetic effects of PDGFRB and MARCH1 identified in GWAS revealing strong associations with semen production traits in Chinese Holstein bulls

Gene

Haplotypes (no.)

SVPE (ml)

SMOT (%)

SCPE (×108/ml)

NSPE (×108)

NMSPE (×108)

PDE3A

H1H1

0.187 ± 0.150

0.807 ± 0.673

−0.240 ± 0.242

0.226 ± 2.307

0.360 ± 1.711

H1H2

−0.191 ± 0.157

−1.106 ± 0.705

−0.384 ± 0.253

−3.121 ± 2.417

−2.108 ± 1.794

H1H3

0.598 ± 0.412

−1.913 ± 1.845

0.589 ± 0.664

11.385 ± 6.326

8.593 ± 4.696

H2H2

−0.222 ± 0.286

−1.125 ± 1.281

0.137 ± 0.461

−0.386 ± 4.394

−0.415 ± 3.261

P-value

0.1266

0.1591

0.4758

0.1878

0.1924

PDGFRB

H1H1

0.110 ± 0.316

−0.438 ± 1.415

−0.151 ± 0.509

0.898 ± 4.852

1.013 ± 3.602

H1H2

0.302 ± 0.223

0.057 ± 1.000

−0.032 ± 0.360

2.976 ± 3.431

2.508 ± 2.547

H1H3

0.089 ± 0.269

0.475 ± 1.203

−0.349 ± 0.433

−1.437 ± 4.127

−0.694 ± 3.064

H1H4

0.149 ± 0.293

0.288 ± 1.313

−0.546 ± 0.473

−1.196 ± 4.502

−0.673 ± 3.342

H2H2

0.237 ± 0.334

0.520 ± 1.494

0.333 ± 0.538

4.645 ± 5.123

3.095 ± 3.802

H2H3

−0.042 ± 0.283

−1.458 ± 1.265

0.191 ± 0.456

2.214 ± 4.340

1.528 ± 3.221

H2H4

−0.119 ± 0.286

−0.541 ± 1.281

−0.073 ± 0.461

−1.039 ± 4.392

−0.638 ± 3.260

H3H3

−0.821 ± 0.598

−1.814 ± 2.676

−1.141 ± 0.964

−13.215 ± 9.179

−9.594 ± 6.814

H3H4

−0.827 ± 0.423

1.172 ± 1.892

−0.400 ± 0.682

−10.118 ± 6.491

−7.527 ± 4.818

H4H4

−0.612 ± 0.568

1.703 ± 2.546

−0.470 ± 0.917

−7.925 ± 8.731

−5.668 ± 6.481

P-value

0.3630

0.9750

0.9280

0.5946

0.6112

MARCH1

H1H1 (231)

0.364 ± 0.171a

0.622 ± 0.768

−0.033 ± 0.276

3.347 ± 2.633

2.557 ± 1.954

H1H2 (149)

0.248 ± 0.213ab

−0.421 ± 0.956

−0.250 ± 0.344

1.502 ± 3.278

1.519 ± 2.433

H1H3 (156)

−0.456 ± 0.210b

−1.372 ± 0.931

−0.346 ± 0.335

−5.223 ± 3.193

−3.711 ± 2.370

H2H2 (38)

0.519 ± 0.423ab

0.987 ± 1.892

0.469 ± 0.682

7.373 ± 6.491

5.449 ± 4.818

H2H3 (58)

−0.664 ± 0.342ab

−1.369 ± 1.532

−0.376 ± 0.552

−7.913 ± 5.254

−5.872 ± 3.900

H3H3 (27)

−1.030 ± 0.501ab

2.910 ± 2.245

−0.443 ± 0.808

−11.030 ± 7.699

−8.129 ± 5.715

p-value

0.0013**

0.4109

0.8877

0.0760

0.0740

  1. P-value is the significance level from analyses of the association of haplotypes with semen production traits. **: P < 0.01. Superscript letters (P < 0.05; Bonferroni-adjusted value after multiple testing) refer to a significant difference among the genotypes