Table 1 Summary of the polymorphic microsatellite loci used in the B. flabellifer population
Primer | Repeat motif | Primer sequence (5′-3′) | Tm (°C) | Allele size (bp) | PIC |
|---|---|---|---|---|---|
ESSR75 | (AAG)5 | F:AGATGGTTGGAGATTTCATGGT R:AACTTGAGGGTGCCATTACAAG | 60 | 270–285 | 0.38 |
175–190 | 0.44 | ||||
ESSR76 | (AGC)5 | F:CCATACCAGCAGAAGAGGATGT R:CTGAAGGTCATAGGGGTCTCTG | 60 | 350–365 | 0.52 |
190–205 | 0.56 | ||||
ESSR82 | (GCT)5 | F:CCCTCGACACCCATAGTTATTT R:CTCGATTTCTGGCCTCTCATAC | 60 | 200–215 | 0.39 |
ESSR332 | (AT)6 | F:AGTTAATGTGTCAGGGCCAGTT R:CTTGGTTCACTTGGGTGTGTC | 60 | 230–242 | 0.42 |
ESSR553 | (A)19 | F:ATAAATTGTGCGAGGGGAAAAC R:AGATCCGCGACAGGTCTTAAC | 60 | 220–239 | 0.37 |
125–144 | 0.34 | ||||
ESSR566 | (AG)7 | F:GTGTCATCAAATTCGGTCCTTT R:CGGTTCTTCTGCTGCTCTACTT | 60 | 240–254 | 0.44 |
125–139 | 0.56 | ||||
ESSR609 | (GA)7 | F:AGGCGGTGATGAAGATGAAG R:CTCCTCTCAAACAGAGTGGGAT | 59 | 150–164 | 0.4 |
ESSR650 | (AG)14 | F:GCCTTTTCTGGTTAATGGACTG R:GTTTGTCTATGGATGATTGTGAGG | 59 | 200–228 | 0.32 |
ESSR652 | (GAG)6 | F:CATACCGTCACCACTCAGAAAC R:GCCGTCATTCTACCAGTTGAG | 60 | 150–168 | 0.33 |
ESSR673 | (GGC)8 | F:TTCTGGCTACGAGCATAAGGA R:TCAATAACCCTGGCTAAACACA | 59 | 150–168 | 0.23 |
75–113 | 0.36 | ||||
ESSR681 | (AAAT)5 | F:TCTGAATTGTCGGAGTGGC R:CATCCTTGCGTAAACAAAAGAG | 59 | 350–370 | 0.49 |
130–150 | 0.56 | ||||
mEgCIR2332 | (GA)14 | F:GAAGAAGAGCAAAAGAGAAG R:GCTAGGTGAAAAATAAAGTT | 55 | 250–278 | 0.38 |
mEgCIR3295 | (GT)7 (GA)23 | F:TGCCTCCAGACAATCAC R:GTAAGGCTTAACCAGATAAC | 55 | 300–360 | 0.62 |
250–310 | 0.16 | ||||
mEgCIR3311 | (GA)15 | F:AATCCAAGTGGCCTACAG R:CATGGCTTTGCTCAGTCA | 55 | 180–210 | 0.31 |
mEgCIR3413 | (GA)18 | F:AAAGCTATGGGGTGAAAGAT R:TGGATAAGGGCGAGAAGAGA | 55 | 350–386 | 0.27 |
250–286 | 0.44 | ||||
mEgCIR3477 | (GA)22 | F:CCTTCAAGCAAAGATACC R:GGCACCAAACACAGTAA | 55 | 250–294 | 0.15 |
mEgCIR3592 | (GA)20 | F:GAGCCAAAACAGACTTCAA R:ACCGTATATGACCCCTCTC | 55 | 230–270 150–190 | 0.46 0.3 |
mEgCIR3755 | (GA)15 | F:GCTCACCAAAAAGTGTTAAGTC R:AGTTTCAACGGCAGGTATAT | 55 | 340–370 140–170 | 0.02 0.1 |
mEgCIR3788 | (GA)18 | F:TTGTATGACCAAAGACAGC R:AGCGCAACATCAGACTA | 55 | 180–216 | 0.43 |