Generation of megabase-scale deletions, inversions and duplications involving the Contactin-6 gene in mice by CRISPR/Cas9 technology

Table 3 Primers used for genotyping F0 offspring with deletions, duplications and inversions

Primers	Sequence (5′ - 3′)	Positions on mouse chromosome 6
FWD1.1	TGGGTCCTTGGAGAGGAACA	chr6: 103,842,509-103,842,528
FWD1.2	ACTCTGGTGACAATGTGCGT	chr6: 103,841,824-103,841,843
REV1	TGCACATGACCCATGACCTC	chr6: 103,842,786-103,842,767
FWD2.1	TCCCCATCTGCTGGCTCTAT	chr6: 104,979,678-104,979,697
FWD2.2	AGAGGTTGATGCAAGCTGCC	chr6: 104,979,540-104,979,559
REV2	CCCCCAAGTGATGCTTCTGT	chr6: 104,980,355-104,980,374
FWD-SNP	TTGCCCTGGTTGTCTTTTATTCAT	chr6: 104,847,831-104,847,854
REV-SNP	AGCACAAACCATGTCACCAAG	chr6: 104,848,314-104,848,334

ISSN: 2730-6844