Table 4 The sequence-based identification of combinations of mutations in the beta-thalassemia specimens and corresponding HRM primer sets

1

c.79 G > A^a and c.92 + 5 G > C^a

P1R1

2

c.92 + 5 G > C^b

P1R1

3

c.47G > A^a and c.79G > A^a

P1R1

4

c.79 G > A^a and c.126_129delCTTT^a

P1R1& P2R2

5

c.46delT^a and c.79G > A^a

P1R1

6

c.27-28insG^a and c.79G > A^a

P1R1

7

c.92 G > C^a and c.92 + 5 G > C^a

P1R1

8

c.79G > A^a and c.92G > C^a

P1R1

9

c.92 + 5 G > C^a and c.92 + 130 G > C^a

P1R1 & P2R2

10

c.92 + 5 G > C^a and c.126_129delCTTT^a

P1R1 & P2R2

11

c.79G > A^b

P1R1

12

c.79G > A^a

P1R1

13

c.79G > A^a and c.92 + 130 G > C^a

P1R1 & P2R2

14

c.33C > A^a and c.51delC^a

P1R1

15

c.79G > A^a and c.126delC^a

P1R1 & P2R2

16

c.126_129delCTTT^b

P2R2

17

c.135delC^a

P2R2