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Table 4 The sequence-based identification of combinations of mutations in the beta-thalassemia specimens and corresponding HRM primer sets

From: High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Sl no. Combinations of mutations Number of samples Primers for HRM
1 c.79 G > Aa and c.92 + 5 G > Ca 55 P1R1
2 c.92 + 5 G > Cb 21 P1R1
3 c.47G > Aa and c.79G > Aa 3 P1R1
4 c.79 G > Aa and c.126_129delCTTTa 3 P1R1& P2R2
5 c.46delTa and c.79G > Aa 2 P1R1
6 c.27-28insGa and c.79G > Aa 2 P1R1
7 c.92 G > Ca and c.92 + 5 G > Ca 2 P1R1
8 c.79G > Aa and c.92G > Ca 2 P1R1
9 c.92 + 5 G > Ca and c.92 + 130 G > Ca 2 P1R1 & P2R2
10 c.92 + 5 G > Ca and c.126_129delCTTTa 2 P1R1 & P2R2
11 c.79G > Ab 1 P1R1
12 c.79G > Aa 1 P1R1
13 c.79G > Aa and c.92 + 130 G > Ca 1 P1R1 & P2R2
14 c.33C > Aa and c.51delCa 1 P1R1
15 c.79G > Aa and c.126delCa 1 P1R1 & P2R2
16 c.126_129delCTTTb 1 P2R2
17 c.135delCa 1 P2R2
  1. a = Indicates heterozygous mutation; b = indicates homozygous mutations