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Table 5 Combinations of mutations identified by HRM and confirmed by sequencing of unknown samples

From: High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Sl no. Combinations of mutations Number of samples Primers for HRM
1 c.79G > Aa and c.92 + 5 G > Ca 23 P1R1
2 c.92 + 5 G > Cb 8 P1R1
3 c.79G > Aa and c.126_129delCTTTa 2 P1R1 & P2R2
4 c.79G > Ab 1 P1R1
5 c.27-28insGa and c.79G > Aa 1 P1R1
6 c.47G > Aa and c.79G > Aa 1 P1R1
7 c.92 + 5G > Ca and c.92 + 130 G > Ca 1 P1R1 & P2R2
8 c.92 + 5G > Ca and c.126_129delCTTTa 1 P1R1 & P2R2
9 c.79G > Aa and c.92G > Ca 1 P1R1
10 c.46delTa and c.79G > Aa 1 P1R1
11 c.92G > Ca and c.92 + 5G > Ca 1 P1R1
  1. a = Indicates heterozygous mutation; b = indicates homozygous mutations