Table 1 Real-time PCR primers and sizes of the amplification products of the target and housekeeping genes
From: RNA-seq analysis of lncRNA-controlled developmental gene expression during puberty in goat & rat
Gene | Forward primer, 5′-3′ | Reverse primer, 5′-3′ | Product size, (bp) |
|---|---|---|---|
XLOC_1041225 | GAGATGATAGCGAGATAAGAGG | GTTAGGTGACATAGTGGTTCC | 113 |
XLOC_1840596 | GCCTACCTAAGATTGAAGCAGTC | CAGTGAGGGAGCGAGAACC | 122 |
XLOC_2050950 | GCCAGCAAGAGGAAGAAGAG | CACAGAGCAGAGTTCACAGG | 105 |
XLOC_777127 | TGATTGTGGACCTCTAAGC | TGCGGACTCATTCTTCTG | 147 |
XLOC_446331 | CTCTGTTGTCTGGTCCTTC | TGCCATTGATGTCCTTACG | 200 |
XLOC_414038 | GGACTGACTGGCTTCTGAG | TGGCTGTGCTGGATTGAC | 140 |
XLOC_181783 | TGCTGATTCTCCCTGTGGTTTATG | GCTTCCCTGGTTGTGCTTGG | 104 |
XLOC_113511 | CTTTCTCCTCCCGCTCTG | TCTGATCTCGCTGTCTTCG | 132 |
Igfbp5 (goat) | CCCAACTGTGACCGCAAAG | TCCACGCACCAGCAGATG | 86 |
Igfbp6 (goat) | TACAAGACACTGAGATGG | CTATGGTCACAATTAGGC | 115 |
Tgfbi (goat) | GTGCCCGCCTGCTGAAAG | TGCTGGATGTTGTTGGTGACG | 94 |
Kiss-1 (goat) | GCCGCTGTTGTTCTGTTGAC | CTGGGGTTCTGCCATTTGA | 117 |
Igfbp5 (rat) | CAGTCGTGTGGCGTCTAC | AGCGGCTTCTCCTCATCC | 77 |
Igfbp6 (rat) | CCGCAGACACTTGGATTCAG | TCACAGTTTGGCACATAGAGC | 87 |
Tgfbi (rat) | CAGTCGTGTGGCGTCTAC | AGCGGCTTCTCCTCATCC | 163 |
Kiss-1 (rat) | TGCTGCTTCTCCTCTGTG | CCAGGCATTAACGAGTTCC | 116 |
Actb (goat) | CGTGACATCAAGGAGAAG | GAAGGAAGGCTGGAAGAG | 171 |
β-actin (rat) | CCCATCTATGAGGGTTACGC | TTTAATTGTCACGCACGATTTC | 150 |