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Fig. 3 | BMC Genetics

Fig. 3

From: A deeper view into the significance of simple sequence repeats in pre-miRNAs provides clues for its possible roles in determining the function of microRNAs

Fig. 3

PCR products showing difference in size of the amplicons observed after Normal PCR with miRNA specific forward and reverse primers and Deletion PCR with forward and reverse primers designed to avoid SSR regions in pre-miRNAs. a Normal PCR with 15 sets of miRNAs (miR164b, miR408, miR2936, miR166e, miR8183, miR167d, miR5021, miR169e, miR166f, miR167c, miR863, miR5015, miR3434, miR156b and miR394a). b and c Primary and secondary deletion PCR with corresponding primer pair combinations; Lane A1, B1, C1:100 bp ladder, lane A17, B12, C7: 1 kb ladder, lane A2 to A16: Normal PCR products with miRNA specific forward and reverse primers; lane B2 to B11: Primary amplicons observed; lane C2 to C6: Final deletion PCR amplicons observed (* corresponds to deletion)

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