Primer names | Primer sequence (5′ to 3′) | Amplicon size (bp) | Exon | Overall outcomes |
---|---|---|---|---|
Hfr-1 | ||||
 Hfr-1_F1 | CCTCAGTCTTTCACCTTGGAGACC | 294 | I | The sequences were poor. |
 Hfr-1_R1 | CCCGAGGTGCAAACCTTGAGC | |||
 Hfr-1_F2 | GGTTTACAGGTTCCAGTTTCAAGG | 205 | II | 7 SNPs occurred randomly across cultivars |
 Hfr-1_R2 | CACCAGAGGTCTTAAGAAGGAGAG | |||
 Hfr-1_F3 | CCGAGTCCGACCAGCTTTCTC | 237 | III | 7 SNPs were detected –some result in no amino acid change, some result in amino acid substitution but did not differentiate any R cultivar from S ones. |
 Hfr-1_R3 | CTACCGTGTCCTTGTTATCCCCAC | |||
 Hfr-1_F4 | CCTTCAGAGATCGTTACGGAAGT | 210 | IV | 8 SNPs were detected. The SNP at nt 883 (AF483596) was found to differentiate some R cultivars from S cultivars (Table 3). |
 Hfr-1_R4 | CTCCACGTAYTTACCAGCGCAC | |||
Hfr-2 | ||||
 Hfr-2_F1 | GCAAGTACCTAGGTAGCGTGC | 340 | I | 2 SNPs were observed to occur in random. |
 Hfr-2_R1 | CGTACCTGATGGATAGATTATTGTCC | |||
 Hfr-2_F2a | CTTCCGTCCYCAGCAGACAAC | 678 | II | 29 SNPs were detected but were found across both S and R cultivars. |
 Hfr-2_R2a | GGTGTCATTGGTTGAAGTCATGCTGG | |||
 Hfr-2_F2b | CTATGGCGTGGAGTTCAAGCTC | 433 | II | The SNP at nt 1294 (GenBank AY587018) was found to differentiate some R cultivars from S cultivars (Table 3). 16 other SNPs found were not informative. |
 Hfr-2_R2b | TCTTGTAGGTAACAACCTCTCCTG |