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Table 2 SNP assays for Hfr-1 and Hfr-2

From: Development of SNP assays for hessian fly response genes, Hfr-1 and Hfr-2, for marker-assisted selection in wheat breeding

Primers and Probes Sequences (5′ to 3′)
SNP883_Hfr-1 assay
 SNP883_Hfr-1_F TTCAGAGATCGTTACGGAAGTTTCTG
 SNP883_Hfr-1_R GTGAGGGATGCTATAGCATTATATTCGA
 SNP883_Hfr-1_probe1 (Vic) ACAGTTGGTGTCTTTG
 SNP883_Hfr-1_probe2 (Fam) ACAGTTGGTATCTTTG
SNP1294_Hfr-2 assay
 SNP1294_Hfr-2_F GGTCATGGAGGCACCAC
 SNP1294_Hfr-2_R GCAGAAGGGAAGGTTGAGGTATC
 SNP1294_Hfr-2_probe1 (FAM) CGTTGTCTGAACGGATGA
 SNP1294_Hfr-2_probe2 (CalFluor) CGTTGTCTGAATGGATGA