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Fig. 2 | BMC Genetics

Fig. 2

From: Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression

Fig. 2

Age-related increased expression in skeletal muscle of aged mice compare to young mice. a Comparisons of the mRNA expression in skeletal muscle (rectus femoris) between wild-type old mice (24 months old) and wild-type young mice (3 months old). Four mice per age. The results are presented as the mean ± SD. p < 0.05; p < 0.005. b Increased expression of genes that are involved in cardiogensis and proliferation of cardiomyctes, such as Gata4, Tbx5, Hand2, and Myocd, as well as their downstream genes, Tnnt2, Tnnc1, Myh6, Myh7, Casq2, Hspb7, Myl4, Myl2, and Tnni1. These data were analyzed by using Ingenuity IPA software. c For the immunohistochemistry staining of Myh6, the brown signal indicates the Myh6 expressed positions. Arrow head indicate myofibers have undergone regenerative process. The black arrows indicate the degenerated myofibers. d Cardiac-specific myosin isoform Myh6 was detected by western blotting analysis. e The levels of nAChR (also known as CHRNA1) were detected in extracted proteins from 3- and 24-month-old rectus femoris. Heat shock protein 70 (HSP70) was used as an internal control. A total of 120 μg of protein from each fraction was loaded

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