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Table 1 Study sample sizes and summary data on transmission ratio distortion (TRD) in polygyne Solenopsis invicta.

From: Unexpected patterns of segregation distortion at a selfish supergene in the fire ant Solenopsis invicta

Sample sizes

Number of nests from which polygyne study queens

12

Number of queens genotyped

113

Number of queens producing study progeniesa

101 (8.4 ± 1.7, 4–11)

Number of loci genotyped in queens / progenies

15

Mean number of progenies genotyped per locusb

93.1 (40–101)

Number of embryos with DNA extracted

3621

Number of embryos successfully genotyped

3512

Number of presumed non-embryonated eggsc

109 (0.030)

Number of embryos genotyped per progenyd

Locus

Mean no.

Bertha

32.7

C27

32.6

C294

32.1

C536

32.3

cassidy

31.7

Gp-9

34.5

i_109

33.6

i_114

32.6

i_120

31.9

i_126

33.1

i_129

30.8

red_ant

34.0

Sol-42f

32.9

Sol-49

32.5

sunrise

33.9

Summary TRD data

Locuse

Number of segregating progeniesf

Number of embryos genotyped in segregating progeniesg

Mean kh

Proportion of segregating progenies with k > 0.65i

Proportion of segregating progenies with k significantly > 0.5j

red_ant

12 (0.30)

417 (34.8)

0.568 (0.549–0.589)

0.000

0.000 (−)k

C27

35 (0.36)

1153 (32.9)

0.558 (0.547–0.571)

0.000

0.000 (0.026, 0.000)l

C536

80 (0.81)

2578 (32.2)

0.564 (0.555–0.573)

0.038

0.025 (0.025, 0.000)

Sol_42f

77 (0.77)

2542 (33.0)

0.566 (0.555–0.577)

0.065

0.039 (0.040, 0.000)

i_114

72 (0.71)

2344 (32.6)

0.565 (0.556–0.576)

0.056

0.042 (0.044, 0.000)

sunrise

50 (0.50)

1700 (34.0)

0.581 (0.566–0.597)

0.100

0.060 (0.063, 0.000)

cassidy

65 (0.70)

2065 (31.8)

0.568 (0.555–0.582)

0.062

0.062 (0.063, 0.000)

i_109

81 (0.80)

2712 (33.5)

0.572 (0.560–0.585)

0.074

0.074 (0.074, 0.000)

i_120

74 (0.74)

2392 (32.3)

0.575 (0.563–0.587)

0.122

0.081 (0.080, 0.000)

Sol_49

76 (0.75)

2464 (32.4)

0.577 (0.565–0.590)

0.092

0.092 (0.094, 0.000)

Bertha

67 (0.66)

2180 (32.5)

0.581 (0.568–0.595)

0.134

0.104 (0.106, 0.034)

i_129

29 (0.30)

851 (29.3)

0.579 (0.556–0.605)

0.138

0.138 (0.140, 0.034)

Gp-9

101 (1.0)

3493 (34.6)

0.584 (0.572–0.595)

0.168

0.168 (0.166, 0.069)

C294

61 (0.98)

1955 (32.0)

0.589 (0.572–0.607)

0.197

0.180 (0.182, 0.069)

i_126

85 (0.84)

2808 (33.0)

0.590 (0.575–0.605)

0.235

0.200 (0.205, 0.103)

  1. The term “progeny” is used to refer to a group of diploid embryos that are the offspring of a single mated polygyne queen (i.e., a family of diploid siblings). Three of the15 loci studied are located within the Sb supergene (see Additional file 4: Table S2 for genomic coordinates of all loci); these supergene loci are highlighted in bold font
  2. aThese queens are a subset of the total 113 queens genotyped. Mean numbers of queens originating from each nest (±standard deviation and range) are in parentheses
  3. bRanges in parentheses
  4. cNon-embryonated eggs fail to undergo embryogenesis and may serve a trophic function. Non- embryonated eggs as a proportion of total eggs studied is in parentheses
  5. dExcludes complete genotyping failures in a progeny; loci are listed in alphabetical order
  6. eLoci are arranged from lowest to highest proportions of segregating progenies that feature k significantly > 0.5 (binomial test)
  7. fSegregating progenies as proportions of total progenies genotyped are in parentheses
  8. gMean number of embryos genotyped per segregating progeny are in parentheses
  9. hIn this table, the parameter k is the proportion of gametes in segregating progenies carrying an allele present at frequencies >0.5 (unpolarized k). The mean as well as the two-tail 95% confidence intervals (in parentheses) are based on 1000 bootstrap replicates
  10. iFor the mean sample sizes of 32-33 embryos genotyped per segregating locus in this study, k=0.65 is a threshold above which segregation ratios generally depart significantly from 1:1 according to one-tailed exact binomial tests
  11. jObserved proportions based on exact binomial tests; bootstrap/rarefaction estimates of the observed proportions, along with their lower one-tailed 95% confidence limits, are shown respectively in parentheses. Confidence limits greater than 0.05 (bold font) indicate significant TRD at a locus
  12. kNumber of segregating progenies was too low to conduct bootstrap analyses
  13. lBootstrap estimates assume that the next progeny sampled would have yielded a k value significantly > 0.05
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BMC Genomic Data

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