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Table 1 The parameters Proteome of Discoverer 1.4

From: Seedless mutant ‘Wuzi Ougan’ (Citrus suavissima Hort. ex Tanaka ‘seedless’) and the wild type were compared by iTRAQ-based quantitative proteomics and integratedly analyzed with transcriptome to improve understanding of male sterility

Item

value

Enzyme

Trypsin

Max Missed Cleavages

2

Fixed modifications

Carbamidomethyl (C), iTRAQ4/8plex (N-term), iTRAQ 4/8plex (K)

Variable modifications

Oxidation (M), iTRAQ 4/8plex (Y)

Peptide Mass Tolerance

± 20 ppm

Fragment Mass Tolerance

0.1 Da

Database pattern

Decoy

Peptide FDR

≤0.01

Protein Quantification

The protein ratios are calculated as the median of only unique peptides of the protein

Experimental Bias

Normalizes all peptide ratios by the median protein ratio. The median protein ratio should be 1 after the normalization.