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Fig. 8 | BMC Genetics

Fig. 8

From: The transcription factor PRO44 and the histone chaperone ASF1 regulate distinct aspects of multicellular development in the filamentous fungus Sordaria macrospora

Fig. 8

Deletion of the asm2 (SMAC_09436) gene leads to defects in ascospore maturation. Δasm2 (ΔSMAC_09436, spore isolate S148783) and a complemented transformant (spore isolate RL726) were grown on full medium (BMM) or defined medium (SWG) at 25 °C for the indicated times. Scale bars are the same for all ascus rosette pictures (left column for each time point) and pictures of spores discharged on the lid of the petri dish (right column for each time point), respectively. In the asm2 deletion strain, many ascospores remain less pigmented even after 14 d, and many asci contain mature and immature spores (red arrows), which does not occur in the wild type where spores within an ascus have the same maturation stage. In contrast to the wild type, which only discharges mature (black) ascospores, the deletion mutant discharges immature ascospores. The complemented transformant shows wild type-like ascus and ascospore maturation, and except for few less pigmented spores (blue arrows), only mature spores are discharged

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