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Fig. 3 | BMC Genetics

Fig. 3

From: The main WAP isoform usually found in camel milk arises from the usage of an improbable intron cryptic splice site in the precursor to mRNA in which a GC-AG intron occurs

Fig. 3

Milk protein profiling by LC-ESI-MS of a Bactrian camel milk from the Shymkent region. Eleven major milk protein fractions were identified from RP-HPLC profile (3.A) in the following order: glycosylated κ-CN A and B (I), non-glycosylated κ-CN A and B (II), WAP (III), shorter (∆ex16 and 13′) + short (∆ex16) isoforms of αs1-CN A and C (IV and V), α-LAC + αs1-CN A and C + (VI), αs2-CN* (VII), PGRP + αs2-CN* (VIII), LPO/CSA (IX), β-CN A and B (X) and γ2-CN A and B (XI). Multicharged-ions spectrum from compounds contained in fraction III (3.B). After deconvolution (3.C) the spectrum shows the presence of cognate camel WAP A-0P (12,546 Da) and 1P (12,644 Da) indicated in black, and molecular masses corresponding to a new WAP variant (named B) without (12,596 Da) and with (12,676 Da) one phosphate group, indicated in red. *Splicing variants of αs2-CN with different phosphorylation levels (Ryskaliyeva et al., submitted)

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