Skip to main content

Table 1 Primer sequences of the target fragments containing the cleavage sites

From: Characterization and functional analyses of the human HTR1A gene: 5’ regulatory region modulates gene expression in vitro

Target fragments Primer sequencesa
BglII -HTR1A(− 1902)F 5’-GAAGATCTCAGGTGAAAGTGTTAGCCTAGC-3’
BglII -HTR1A(− 1781)F 5’-GAAGATCTCGAGCGCAAGAGGGCACTA-3’
BglII -HTR1A(−1649)F 5′- GAAGATCTTCGGCATAACGGTTTCCAGATG-3’
BglII -HTR1A(−1550)F 5’-GAAGATCTTCTGAGATTAAGAGAGGCTAGCCG-3’
BglII -HTR1A(−1409)F 5’-GAAGATCTATCCCTGAATCTACTAGCCACA-3’
BglII -HTR1A(−1381)F 5’-GAAGATCTTGGGAAGTGGCAGTGTCACTG-3’
BglII -HTR1A(− 1235)F 5’-GAAGATCTATTTCGTTCTCTCCCGGTTCC-3’
BglII -HTR1A(−1196)F 5’-GAAGATCTTCACAGGCAATATTCTCCCTGAG-3’
NheI -HTR1A(−1124)F 5’-CTAGCTAGCTTGTCGTCGTTGTTCGTTTG-3’
BglII -HTR1A(−1064)F 5’-GAAGATCTGTGTAATGGTGCGAGAACGG-3’
BglII -HTR1A(−908)F 5’-GAAGATCTGGATGCTGACACGATTTAAGAAT-3’
BglII -HTR1A(−722)F 5’-GAAGATCTAGACTTGAATGCAAAGACGCTG-3’
HindIII -HTR1A(+ 99)R 5’-CCCAAGCTTGTCGGAGATACCAGTAGTGTT-3’
HindIII -HTR1A(−119)R 5’-CCCAAGCTTCAGGAAGTTCTTACTGCTTC-3’
HindIII-HTR1A(−372)R 5’-CCCAAGCTTAAATATCTAGAACCGAGAAGCC-3’
  1. aThe 5′ end of the primer sequences contained the BglII, HindIII, and NheI restriction enzyme cleavage sites
  2. The numbers in brackets represent the 5′ end position in the HTR1A gene as ATG + 1. F, Forward; R, Reverse