Table 1 Primer sequences of the target fragments containing the cleavage sites
Target fragments | Primer sequencesa |
|---|---|
BglII -HTR1A(− 1902)F | 5’-GAAGATCTCAGGTGAAAGTGTTAGCCTAGC-3’ |
BglII -HTR1A(− 1781)F | 5’-GAAGATCTCGAGCGCAAGAGGGCACTA-3’ |
BglII -HTR1A(−1649)F | 5′- GAAGATCTTCGGCATAACGGTTTCCAGATG-3’ |
BglII -HTR1A(−1550)F | 5’-GAAGATCTTCTGAGATTAAGAGAGGCTAGCCG-3’ |
BglII -HTR1A(−1409)F | 5’-GAAGATCTATCCCTGAATCTACTAGCCACA-3’ |
BglII -HTR1A(−1381)F | 5’-GAAGATCTTGGGAAGTGGCAGTGTCACTG-3’ |
BglII -HTR1A(− 1235)F | 5’-GAAGATCTATTTCGTTCTCTCCCGGTTCC-3’ |
BglII -HTR1A(−1196)F | 5’-GAAGATCTTCACAGGCAATATTCTCCCTGAG-3’ |
NheI -HTR1A(−1124)F | 5’-CTAGCTAGCTTGTCGTCGTTGTTCGTTTG-3’ |
BglII -HTR1A(−1064)F | 5’-GAAGATCTGTGTAATGGTGCGAGAACGG-3’ |
BglII -HTR1A(−908)F | 5’-GAAGATCTGGATGCTGACACGATTTAAGAAT-3’ |
BglII -HTR1A(−722)F | 5’-GAAGATCTAGACTTGAATGCAAAGACGCTG-3’ |
HindIII -HTR1A(+ 99)R | 5’-CCCAAGCTTGTCGGAGATACCAGTAGTGTT-3’ |
HindIII -HTR1A(−119)R | 5’-CCCAAGCTTCAGGAAGTTCTTACTGCTTC-3’ |
HindIII-HTR1A(−372)R | 5’-CCCAAGCTTAAATATCTAGAACCGAGAAGCC-3’ |