Evaluation of fecal samples as a valid source of DNA by comparing paired blood and fecal samples from American bison (Bison bison)

Table 1 List of 15 STR loci chosen due to their high fidelity and efficiency

Marker	Percent matching (excluding reruns)	Percent matching (including reruns)	Chromosome	Major allele frequency (x_i)	Major genotypic frequency (x_i²)
BM7145	100%	100%	1	0.800	0.640
BM4307	100%	100%	1	0.782	0.612
BM2113	96%	96%	2	0.360	0.129
CSSM42	100%	100%	2	0.607	0.368
AGLA293	100%	98%	5	0.958	0.917
SPS113	98%	98%	10	0.613	0.376
BL1036	100%	100%	14	0.313	0.098
BM4513	100%	98%	14	0.872	0.761
BM1706	100%	100%	16	0.660	0.436
BM1225	100%	100%	20	0.433	0.187
BM4107	100%	100%	20	0.363	0.132
BM1905	98%	98%	23	0.495	0.245
BM47	98%	98%	23	0.729	0.531
ILSTS102	96%	96%	25	0.531	0.282
BMS510	96%	96%	28	0.466	0.217

The major allele frequency for each STR was determined based on an extensive library of 10–17,000 plains bison, and used to estimate the probability of identity (2.318 × 10^− 6)

ISSN: 2730-6844