Fig. 2

α-dCNDP2 antibodies are specific to the target protein. a-e Western blots showing that the α-dCNDP2 antibodies recognize a single band migrating slightly lower than the 55 kDa standard. This band is likely to correspond to the 53.2 kDa isoform A. a S2 cells; b embryos; c ovaries; d larval brains; e salivary glands. L3, third instar larval stage. Note that in 4–6 h old embryos the antibodies recognize a second faint band that might correspond to the 47.9 kDa isoform C. f Immunostaining of dCNDP2 depleted (dCNDP2 RNAi) and control S2 cells with α-dCNDP2 antibodies. Scale bar, 20 μm