Table 2 Chs copy-specific primers designed in the current study
From: Organization and evolution of the chalcone synthase gene family in bread wheat and relative species
Purpose | Name | Sequence | Amplicon lengh (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
Copy identification, chromosomal assignment and mapping, RT-PCR | Chs-A1 | Forward 5’TTGTATTCTGCACCACCTCG3’ | 265 | 62 |
|  | Reverse 5’AAGAGTGCCTGACCTACCA3’ | 62 | ||
Chs-B1 | Forward 5’TCGTCGTCGTGGAGGTT3’ | 256 | 56 | |
|  | Reverse 5’TCGGAGCAGACCACCA3’ | 56 | ||
Chs-B2 | Forward 5’ATGTATCAGCAGGGCTGT3’ | 279 | 54 | |
|  | Reverse 5’CTCTGAGTCCGGCAGAAT3’ | 54 | ||
Chs-B3 | Forward 5’GAAGAGGTACATGCACCTG3’ | 481 | 53 | |
|  | Reverse 5’CGCACCGACGATCACG3’ | 57 | ||
Chs-D1 | Forward 5’GATCACCCACCTTGTATT3’ | 257 | 49 | |
|  | Reverse 5’CGCCTGACCTACCAGT3’ | 54 | ||
Chs-A3 group-specific | Chs_gs_A3 | Forward 5’CCCACCTCGACTCGCTA3’ | 409 | 56 |
|  | Reverse 5’GCACTTGACATGTTGCCATATT3’ | 54 | ||
Sequensing | T.durum_A1 | Forward_1 5’AGGAAGAGGTACATGCACCTT3’ | 567 | 56 |
|  | Reverse_1 5’GATGGCACCCTCTGAGTCT3’ | 56 | ||
|  | Forward_2 5’CGCTGGTAGGTCAGGCA3’ | 543 | 57 | |
|  | Reverse_2 5’CTGGGACACTATGGAGGACAA3’ | 56 | ||
Reference | HvActin | Forward 5’TCGCAACTTAGAAGCACTTCCG3’ | 130 | 60 |
|  | Reverse 5’AAGTACAGTGTCTGGATTGGAGGG3’ | 60 |