Table 1 Gene-specific primers used in current study
From: Genetic control of anthocyanin pigmentation of potato tissues
Gene | GenBank ID | Purpose | Annealing temperature (°C) | Forward primer (FP, 5′ → 3′) | Reverse primer (RP, 5′ → 3′) | Primer binding site | |
|---|---|---|---|---|---|---|---|
FP | RP | ||||||
StAN1 | KM822778 AY841130 AY841128 KM822780 | diagnostic PCR, sequencing | 55 | GGAAGGACAGCTAACGATG | AGTGTTCTTTCAGCTTCTCC | E3 | E3 |
chr10:51745200–51,749,200 | PCR, sequencing | 50 | GTCACATCACTACACCACAT | TCCACTTCATCCCAATCAAAG | promoter | E2 | |
AY841128 | full length CDS amplification, sequencing | 50 | ATGACTTCACATGTAATGATCAT | CTAATTAAGTAGATTCCATATATCA | E1 | E3 | |
AY841128 | qRT-PCR (a) | 60 | GGAGAAGGAAAGTGGCATCTTGTTCCA | TCCACTTCATCCCAATCAAAG | E1 | E2 | |
AY841128 | qRT-PCR (b) | 60 | GAGAAGCTGAAAGAACACTACCT | CACCATCACCTTGTCCTTGT | E3 | E3 | |
StMYBA1 | JQ219855 | qRT-PCR | 60 | GTGGTCACTTATTGCTGGTAGA | GGCGAGGAGGAGGAGTAATA | E3 | E3 |
StMYB113 | KU242748 | qRT-PCR | 60 | CTAGGCAACAGATGGTCACTTAT | CTTCCTGTGTAGGTGTGTGTT | E3 | E3 |
StbHLH1 | JX848660 | qRT-PCR | 60 | CCACCAAAGCCAGCTTTATC | ATCCGCTGGACAAATACCAG | E5 | E5 |
StJAF13 | KP317176 | qRT-PCR | 60 | CTGCAGAGCAGACATCTGATAA | GCAGCTTTCAGGTTCCATTTC | E7 | E7 |
StWD40 | JX848661 | qRT-PCR | 60 | ACCCTTAAGCCTGTTCCAAATC | CACCGGAAGAGGCAAGAATATC | E1 | E1 |