Markers are validated with 20 commercial early- and late-flowering cabbage lines, with ‘F7R7’ marker; of BoFLC1.C9 gene matched 80% with the lines, the heterozygous lines (lanes: 10, 11, 13, and 16) are not matched with the phenotypes exactly. Here, P1 = late-flowering parent (BN3848), P2 = early-flowering parent (BN623), F1 = (BN3848 × BN623), M = 100 bp DNA marker, Red color numbered (10 early-flowering lines): lane 1, 2, 4, 7, 9, 11, 12, 15,17 are 17 FLE1, 17 FLE2, 17 FLE3, 17 FLE4, 17 FLE5, 17 FLE6, 17 FLE7, 17 FLE8, 17 FLE9, and 17 FLE10, respectively and black color numbered (10 late-flowering lines): lanes 3, 5, 6, 8, 10, 13, 14, 16,19 and 20 are 17 FLL1, 17 FLL2, 17 FLL3, 17 FLL4, 17 FLL5, 17 FLL6, 17 FLL7, 17 FLL8, 17 FLL9, and 17 FLL10, respectively