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Table 3 Multiplex and reaction conditions for optimized white-tailed deer microsatellite panel

From: Identification and evaluation of a core microsatellite panel for use in white-tailed deer (Odocoileus virginianus)

Locus

Primer Sequence

Motif

Vol

Dye

AT

Multiplex 1

 RT9

F: TGAAGTTTAATTTCCACTCT

R: CAGTCACTTTCATCCCACAT

2

0.20

6-FAM

57.0

 BM4107

F: AGCCCCTGCTATTGTGTGAG

R: ATAGGCTTTGCATTGTTCAGG

2

0.18

6-FAM

57.0

 P

F: TTTCACTGTTTTCTCCTTCAGA

R: TGCCCAATCAGATGTTGTAG

4

0.20

NED

57.0

 Cervid 1†

F: AAATGACAACCCGCTCCAGTATC

R: TCCGTGCATCTCAACATGAGTTAG

2

0.15

NED

64.0

Multiplex 2

 Q

F: AATGTGTCAGTGAAGGTCTTC

R: ATCCAGGCAACCATCTAG

4

0.18

6-FAM

60.0

 RT5

F: CAGCATAATTCTGACAAGTG

R: GTTGAGGGGACTCGACTG

2

0.16

6-FAM

60.0

 BL42†

F: ACAAGTCAAGGTCAAGTCCAAATGCC

R: CGATTTTTGTGTTAATTTCATGC

2

0.20

PET

54.0

Multiplex 3

 RT7

F: CCTGTTCTACTCTTCTTCTC

R: ACTTTTCACGGGCACTGGTT

2

0.15

VIC

55.0

 BM6438

F: TTGAGCACAGACACAGACTGG

R: ACTGAATGCCTCCTTTGTGC

2

0.17

NED

55.0

 INRA011

F: CGAGTTTCTTTCCTCGTGGTAGGC

R: GCTCGGCACATCTTCCTTAGCAAC

2

0.17

PET

55.0

 OarFCB193

F: TTCATCTCAGACTGGGATTCAGA

R: GCTTGGAAATAACCCTCCTGC

2

0.18

NED

55.0

  1. All primers were multiplexed for PCR and fragment analysis steps with the exception of Cervid 1 and BL42 (†). Polymerase chain reactions for these two loci were run separately and then multiplexed for fragment size analysis. Primers are grouped by multiplex with Motif = expected repeat motifs, Vol = volume of primer mix (μL) for 10 μL reaction volume consisting of equal parts 20 μM forward and reverse primer, Dye = dye color, and AT = annealing temperature (°C)