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Fig. 4 | BMC Genetics

Fig. 4

From: Bos taurus–indicus hybridization correlates with intralocus sexual-conflict effects of PRDM9 on male and female fertility in Holstein cattle

Fig. 4

Ambiguous trace chromatograms associated with heterozygosity for the dairy and beef forms of PRDM9. On top, the ambiguous trace chromatogram was obtained by sequencing of the PCR product amplified from sire #5228 that carries haplotypes #9 and #15 (Table 3). Sequencing was performed using the reverse primer (primer 1, Additional file 1: Table S1). Phased nucleotides and their corresponding encoded protein translations are presented below this chromatogram. Further below, chromatograms were obtained from bacterial-cloned fragments amplified from sire #5611 that carries haplotypes #8 and #10 using the SP6 primer

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