From: The use of the SLC16A1 gene as a potential marker to predict race performance in Arabian horses
Identified SNP | Genetic variant | Method | Primers | Details |
---|---|---|---|---|
C > T Exon 5 NM_001081791.2:g.55601543C > T ENSECAP00000019149.1:p.Val432Ile ss#3021042926 | Missens variant Val/Ile | PCR-RFLP | F CAGCTTTGTTTGGGAAAGGA R CACACCGGTCTCAACCTCTT | PCR-AmpliTaq360 (Applied Biosystem); anneling temp. 57 °C PCR-RFLP with BsrDI endonuclease, alleles: C - 248 bp T – 133, 115 bp |
T > G 5’UTR NM_001081791.2:g.55589063 T > G ss#3021042925 | UTR variant | PCR-HRM | F TGTTGGGGGTGCTTTATTGT R GGGGGTACTTACCTCCACCA | PCR-HRM with the use KAPA HRM FAST PCR Kits (Kapa Biosystem) on QuantStudio 7 Flex (Applied Biosystem) – 323 bp |