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Table 1 The details of methods used to genotype both identified mutations

From: The use of the SLC16A1 gene as a potential marker to predict race performance in Arabian horses

Identified SNP

Genetic variant

Method

Primers

Details

C > T

Exon 5

NM_001081791.2:g.55601543C > T ENSECAP00000019149.1:p.Val432Ile

ss#3021042926

Missens variant Val/Ile

PCR-RFLP

F CAGCTTTGTTTGGGAAAGGA

R CACACCGGTCTCAACCTCTT

PCR-AmpliTaq360 (Applied Biosystem); anneling temp. 57 °C

PCR-RFLP with BsrDI endonuclease, alleles:

C - 248 bp

T – 133, 115 bp

T > G

5’UTR

NM_001081791.2:g.55589063 T > G

ss#3021042925

UTR

variant

PCR-HRM

F TGTTGGGGGTGCTTTATTGT

R GGGGGTACTTACCTCCACCA

PCR-HRM with the use KAPA HRM FAST PCR Kits (Kapa Biosystem) on QuantStudio 7 Flex (Applied Biosystem) – 323 bp