Table 1 Functional evaluation of ItfWRKYs under abiotic stress
Function known WRKYs related abiotic stress | Homologous WRKYs in I. trifida | |||||
|---|---|---|---|---|---|---|
Species | Protein name | Function | Reference | Protein name | Protein identify (%) | Gene expression under abiotic stress |
Arabidopsis | AtWRKY25 | Overexpression enhanced heat resistance and confer salt tolerance | [14] | ItfWRKY28.1 | 50.59 | Under heat ↑ Under salt ↑ |
|  | AtWRKY26 | Overexpression enhanced heat resistance | [14] | ItfWRKY43.1 | 51.69 | Under heat ↑ |
|  | AtWRKY33 | High-temperature represses its expression and induces the expression of AtWRKY25 and AtWRKY26; and confer salt tolerance | [14] | ItfWRKY13.3 | 52.99 | Under heat ↓ Under salt ↑ |
|  | AtWRKY34 | Negative regulator in pollen specific cold response | [16] | ItfWRKY38 | 49.34 | Under cold ↑ |
|  | AtWRKY39 | Overexpression enhanced heat stress resistance of transgenic plants | [17] | ItfWRKY44.2 | 51.30 | Under heat ↑ |
|  | AtWRKY57 | Induced by drought and its expression increase Arabidopsis drought tolerance | [18] | ItfWRKY4 | 84.93 | Under drought ↑ |
Triticum aestivum | TaWRKY1 | Confer drought and/or heat resistance in Arabidopsis | [18] | ItfWRKY65 | 64.00 | Under drought ↓ Under heat ↑ |
|  | TaWRKY2 | Confer transgenic plants tolerance to drought stress | [69] | ItfWRKY13.1 ItfWRKY13.3 | 49.49 49.49 | Under drought ↓ Under drought ↓ |
|  | TaWRKY10 | Overexpression enhanced the tobacco drought and salt tolerance | ItfWRKY41 | 65.17 | Under salt ↑ Under drought ↑ | |
|  | TaWRKY19 | Confer transgenic plants tolerance to drought stress | [69] | ItfWRKY79 | 48.08 | Under drought ↑ |
|  | TaWRKY33 | Confer drought and/or heat resistance in Arabidopsis | [18] | ItfWRKY67 | 41.43 | Under drought ↑ Under heat ↑ |
|  | TaWRKY44 | Confers transgenic tobacco multiple abiotic stress tolerances. | [70] | ItfWRKY79 | 50.44 | Under salt ↑ Under drought ↑ Under heat ↑ Under cold ↑ |
Oryza.sativa L | OsWRKY11 | Transgenic lines showed significant heat and drought tolerance | [71] | ItfWRKY64 | 78.16 | Under heat ↓ Under drought ↑ |
|  | OsWRKY45 | Overexpression enhances salt and drought tolerance | ItfWRKY70 | 48.61 | Under salt ↓ Under drought ↓ | |
|  | OsWRKY72 | Overexpression enhances salt and drought tolerance | ItfWRKY77 | 86.96 | Under salt ↑ Under drought ↑ | |
Glycine max | GmWRKY13 | Transgenic plants increased sensitivity to salt and drought stress while decreased sensitivity to ABA | [28] | ItfWRKY46 | 63.89 | Under salt ↑ Under drought ↑ |
|  | GmWRKY21 | Confer transgenic plant tolerance to cold stress | [28] | ItfWRKY41 | 66.22 | Under cold ↑ |
|  | GmWRKY27 | Improved salt and drought tolerance by inhibiting expression of downstream gene GmNAC29 | ItfWRKY15.2 | 53.74 | Under salt ↑ Under drought ↓ | |
|  | GmWRKY54 | Confer transgenic Arabidopsis plants tolerance to salt and drought | [28] | ItfWRKY64 | 75.61 | Under salt ↑ Under drought ↑ |
Gossypium hirsutum | GhWRKY17 | Overexpression increases Nicotiana tolerance to drought and salt stress | [24] | ItfWRKY30 | 63.49 | Under drought ↓ Under salt ↓ |
|  | GhWRKY25 | Overexpression plants increased salt tolerance but reduced drought tolerance of A. thaliana | [26] | ItfWRKY19 | 78.57 | Under salt ↑ Under drought ↓ |
|  | GhWRKY68 | Reduces resistance to salt and drought in transgenic Nicotiana benthamiana | [75] | ItfWRKY64 | 75.58 | Under salt ↑ Under drought ↑ |
Dendranthma grandiflorum | DgWRKY1 | Overexpression enhances salt tolerance | [74] | ItfWRKY77 | 85.56 | Under salt ↑ |
|  | DgWRKY3 | Overexpression enhances salt tolerance | [74] | ItfWRKY70 | 65.59 | Under salt ↓ |
Boea hygrometria | BhWRKY1 | Bind to BhGolS1 to activate the regulation of BhGolS1 under drought stress | [27] | ItfWRKY15.1 | 56.52 | Under drought ↑ |
Vitis vinifera | VvWRKY24 | Be induced by cold treatment at all-time points | [15] | ItfWRKY49 | 76.32 | Under cold ↑ |
Zea mays | ZmWRKY23 | Enhance tolerance to salt stress | [25] | ItfWRKY72 | 50.00 | Under salt ↓ |
Brassica campestris ssp | BcWRKY46 | Overexpression increases A. thaliana tolerance to drought and salt stress | [73] | ItfWRKY30 | 55.74 | Under salt ↓ Under drought ↓ |