Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

Table 1 Primers used in this study

Primer	Sequence (5′-3′)	explanation	predicted PCR product Size
TaGS3-4A-F	CGATCCTTCTCTGCGGCAAG	primers for amplifying TaGS3-4A	2435 bp
TaGS3-4A-R	CCTACAGACCGACGACTTCCTG
TaGS3-7A-F	CGACTTCCTGTCTCCTTCCGG	primers for amplifying TaGS3-7A	2389 bp
TaGS3-7A-R	TCATGCCCGTCAAAAACACCAG
TaGS3-7D-F	GACGACTTCCTGTCTCCTACTTCC	primers for amplifying TaGS3-7D	2409 bp
TaGS3-7D-R	TCATGCCCGTCAAAAACACCAG
TaGS3-7A-1907-F1	FAM-GCGCCGGTTGCTCCTCATCTTGCG	KASP marker for distinguishing A/G allele
TaGS3-7A-1907-F2	HEX-GCGCCGGTTGCTCCTCATCTTGCA
TaGS3-7A-1907-CR	AGGGACGCCRCCGCAGCACACGGT

ISSN: 2730-6844