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Table 2 Nucleotide sequences of primers used for CDS cloning, 3′ RACE and RT-qPCR in this study

From: Comparative analyses of the Sox9a-Amh-Cyp19a1a regulatory Cascade in Autotetraploid fish and its diploid parent

Primer name

Nucleotide sequence (5′ to 3′)

For CDS cloning

Sox9a-F1

ATGAATCTACTAGACCCCTACCT

Sox9a-R1

GCTGCTGTGCCCAATGCTG

Amh-F1

ATGCTCTTCCACGCAGGATTTTG

Amh-R1

CAAAGAGCAAAAGGAGGGTGTCA

Cyp19ala-F1

ATGGCAGGTGAACTTCTCCAGCC

Cyp19ala-R1

GAGGGCGTTTCTGGGGATGAGC

For 3′ RACE

Sox9a-3′-F

CAACTCCTATTACACCCACGCT

Amh − 3′-F

CAACAACCACGCCATCCTC

Cyp19ala − 3′-F

TGCTCAAACAGAATCCAGAC

 3′-adaptor Primer

CTGATCTAGAGGTACCGGATCC

For RT-qPCR

β-actin-F

CATCTACGAGGGTTACGCCC

β-actin-R

AATTTCCCTCTCGGCTGTGG

Sox9a-F2

TCAATACCCGCACCTCCACAACGCC

Sox9a-R2

TGAAGATGGCGTTCGGGGAGATGTG

Amh-F2

GGAATTCACCAGTCCTGATAGC

Amh-R2

CTGCAGAAGTTCTTGAGTGACG

Cyp19ala-F2

TTGACACCTGGCAGACGGTA

Cyp19ala-R2

CTGCGATTATCATCTCCAACAC

  1. CDS coding sequence, RACE rapid amplification of cDNA ends, RT-qPCR real-time quantitative polymerase chain reaction