Table 1 Genetic characteristics of analysed loci (upper half) and sites (lower half). Number of alleles per locus (N_a), the size range of each locus, allelic richness, unbiased expected heterozygosity (H_e), observed heterozygosity (H_o), fixation index (F_IS), deviations from HW equilibrium, total number of analysed samples (n), number of males and females, number of sequenced samples and haplotypes, haplotype diversity (h) and nucleotide diversity (π) for each site and riverside, respectively

Locus

N_a

Size range [bp]

Allelic richness

H_e

H_o

F_IS

Sites out of HWE

Js 22

7

172–186

2.670

0.496

0.483

0.027

Js 183

11

134–144

4.373

0.794

0.759

0.044

Js 188

15

182–201

4.542

0.789

0.759

0.039

TB 8

4

404–420

2.831

0.621

0.595

0.043

NA, ND

TB 14

36

457–569

7.115

0.966

0.482

0.503**

all, exc. SI

TB 15

31

284–344

6.601

0.942

0.905

0.040

TB 16

16

170–203

4.174

0.697

0.655

0.060

TB 18

32

408–544

6.452

0.933

0.888

0.049*

Site

n

Males/Females

Allelic richness

H_e

H_o

F_IS

Sequences/Haplotypes

h

π

Area [km²]

NA

11

6 / 5

4.911

0.725

0.727

−0.004

8 / 6

0.893

0.008

22.17

NB

18

10 / 8

5.105

0.788

0.786

0.003

7 / 4

0.714

0.007

41.60

NC

5

4 / 1

4.571

0.698

0.771

−0.119

2 / 2

1.000

0.012

9.66

ND

17

9 / 8

5.013

0.731

0.714

0.024

10 / 5

0.800

0.008

73.17

North

51

29 / 22

4.962

0.761

0.748

0.017

27 / 11

0.889

0.009

146.60

SE

11

8 / 3

4.257

0.740

0.753

−0.019

6 / 3

0.733

0.005

47.99

SF

21

9 / 12

3.902

0.685

0.674

0.018

7 / 4

0.810

0.005

1.25

SG

29^a

14 / 14

3.888

0.689

0.714

−0.037

17 / 3

0.581

0.004

17.14

SI

4

3 / 1

3.286

0.628

0.571

0.103

3 / 2

0.667

0.012

16.14

South

65^a

34 / 30

3.895

0.718

0.699

0.026

33 / 6

0.695

0.006

82.52

All

116^a

63 / 52

4.414

0.753

0.720

0.044**

60 / 14

0.878

0.008

229.12