Table 3 Number of individuals used to make DNA pools for each family for BSA-ddRAD library construction followed by number of individuals for each six GIFT families, and GIFT and Stirling broodstock for individual analysis with LG23-linked DNA markers
From: Sex determination in the GIFT strain of tilapia is controlled by a locus in linkage group 23
| Â | Â | BSA-ddRADseq analysis | LG23-linked marker analysis | |
|---|---|---|---|---|
ID | Strain | Individuals in male progeny pool | Individuals in female progeny pool | Individuals analysed |
Family 1 | Stirling | 24 | 24 | Â |
Family 2 | Stirling | 29 | 29 | Â |
Family 1 | GIFT | 25 | 25 | 40 |
Family 2 | GIFT | 30 | 30 | 40 |
Family 3 | GIFT | 30 | 30 | 40 |
Family 4 | GIFT | 30 | 30 | 40 |
Family 5 | GIFT | 30 | 30 | Â |
Family 6 | GIFT | 30 | 30 | Â |
Family 7 | GIFT | 15 | 15 | 30 |
Family 8 | GIFT | 18 | 18 | Â |
Family 9 | GIFT | 28 | 28 | Â |
Family 10 | GIFT | 15 | 15 | Â |
Family 11 | GIFT | 21 | 21 | Â |
Family 12 | GIFT | 22 | 22 | Â |
Family 13 | GIFT | 30 | 30 | Â |
Family 14 | GIFT | 15 | 15 | Â |
Family 15 | GIFT | 22 | 22 | Â |
Family 16 | GIFT | 17 | 17 | Â |
Family 17 | GIFT | 23 | 23 | Â |
Family 18 | GIFT | 23 | 23 | Â |
Family 19 | GIFT | 30 | 30 | 40 |
Broodstock | GIFT | Â | Â | 50 |
Broodstock | Stirling | Â | Â | 4 |