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Fig. 6 | BMC Genetics

Fig. 6

From: Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation

Fig. 6

Hynobius lichenatus p53 protein exhibits inhibitory effects on mammalian cell proliferation. a hyp53-HA protein expression in hyp53-HA/HEK293 stable cell line. The original western blot image is shown in Additional file 5B. b Number of viable hyp53-HA/HEK 293 or GFP-HA/HEK293 stable cells after 2–3 days of incubation following serum starvation for 24 h. The data are expressed as mean ± SD (n = 4). **P < 0.01. The data shown are representative of three independent experiments. c Top: Number of viable hyp53-HA/HEK 293 or GFP-HA/HEK293 stable cells after 7 days of incubation. The data are expressed as mean ± SD (n = 4). **P < 0.01. Bottom: Phase-contrast images of the hyp53-HA/HEK 293 and GFP-HA/HEK293 stable cell lines after 7 days of incubation. The double-headed arrow shows the distance from the proliferating cell front to the side of the plate well. The data shown are representative of three independent experiments

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