Fig. 3
From: Knock-out of TERMINAL FLOWER 1 genes altered flowering time and plant architecture in Brassica napus
Sequence variations in CRISPR/Cas9-induced BnaTFL1s mutants. a The structure of BnaTFL1 genes including four exons (Gray box) separated by three introns (represented by the solid line). The vertical lines in the genes indicate the target sites of sgRNAs, and the arrows indicate directions of the sgRNAs. The red characters indicate the PAM sites in the target sequences. The single-side arrows indicate primers for RT-PCR. The primer pairs of S1/S2, S3/S4, S5/S6, S7/S8 and S9/S10 were used to amplify BnaC02.TFL1, BnaA10.TFL1, BnaA02.TFL1, BnaC03.TFL1 and BnaC09.TFL1, respectively. b Sequences variations at the gRNA target sites of BnaTFL1.A10 (L46) and BnaTFL1.C03 (K70) in the T0, T1 and T2 generations. Blue characters indicate nucleotide insertions, and i1 means one nucleotide insertion. Ref stands for reference sequence. WT stands for wild-type allele. “A1” and “A2” represent the mutated alleles of the target Bnatfl1 genes