Table 1 Genotypic analysis of BnaTFL1s mutants (BnaA02.TFL1, BnaA10.TFL1, and BnaC02.TFL1.) mutagenized by sgRNA1/2 in the T1 and T2 generations
From: Knock-out of TERMINAL FLOWER 1 genes altered flowering time and plant architecture in Brassica napus
Lines | Generations | BnaA02.TFL1 | BnaA10.TFL1 | BnaC02.TFL1 | Â | |||
|---|---|---|---|---|---|---|---|---|
sgRNA1 | sgRNA2 | sgRNA1 | sgRNA2 | sgRNA1 | sgRNA2 | Early flowering | ||
L46 | T0 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L46–1 | T1 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L46–1-1 | T2 | WT | WT | Homozygous (i1–1/i1–1) | WT | WT | WT | No |
L46–1-2 | T2 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L46–1-3 | T2 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L46–1-4 | T2 | WT | WT | Homozygous (i1–1/i1–1) | WT | WT | WT | No |
L69 | T0 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L69–1 | T1 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L69–1-1 | T2 | WT | WT | Homozygous (i1–1/i1–1) | WT | WT | WT | No |
L69–1-2 | T2 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L69–1-3 | T2 | WT | WT | Bi-allelic (i1–1/i1–2) | WT | WT | WT | No |
L69–1-4 | T2 | WT | WT | Homozygous (i1–1/i1–1) | WT | WT | WT | No |
L98 | T0 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |
L98–1 | T1 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |
L98–1-1 | T2 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |
L98–1-2 | T2 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |
L98–1-3 | T2 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |
L98–1-4 | T2 | WT | Homozygous (d1–1/d1–1) | WT | WT | WT | WT | No |